diff --git a/resources/test/fixtures/isort/preserve_tabs_2.py b/resources/test/fixtures/isort/preserve_tabs_2.py
new file mode 100644
index 0000000000..2abfd4afca
--- /dev/null
+++ b/resources/test/fixtures/isort/preserve_tabs_2.py
@@ -0,0 +1,13 @@
+from numpy import (
+	cos,
+	int8,
+	int16,
+	int32,
+	int64,
+	sin,
+	tan,
+	uint8,
+	uint16,
+	uint32,
+	uint64,
+)
diff --git a/src/rules/isort/mod.rs b/src/rules/isort/mod.rs
index 133105e4f2..cbdcc92558 100644
--- a/src/rules/isort/mod.rs
+++ b/src/rules/isort/mod.rs
@@ -715,6 +715,7 @@ mod tests {
     #[test_case(Path::new("preserve_import_star.py"))]
     #[test_case(Path::new("preserve_indentation.py"))]
     #[test_case(Path::new("preserve_tabs.py"))]
+    #[test_case(Path::new("preserve_tabs_2.py"))]
     #[test_case(Path::new("relative_imports_order.py"))]
     #[test_case(Path::new("reorder_within_section.py"))]
     #[test_case(Path::new("separate_first_party_imports.py"))]
diff --git a/src/rules/isort/rules/organize_imports.rs b/src/rules/isort/rules/organize_imports.rs
index d310647533..74d2a7e2cf 100644
--- a/src/rules/isort/rules/organize_imports.rs
+++ b/src/rules/isort/rules/organize_imports.rs
@@ -1,8 +1,9 @@
 use std::path::Path;
 
+use itertools::{EitherOrBoth, Itertools};
 use ruff_macros::derive_message_formats;
 use rustpython_ast::{Location, Stmt};
-use textwrap::{dedent, indent};
+use textwrap::indent;
 
 use super::super::track::Block;
 use super::super::{comments, format_imports};
@@ -43,6 +44,17 @@ fn extract_indentation_range(body: &[&Stmt]) -> Range {
     Range::new(Location::new(location.row(), 0), location)
 }
 
+/// Compares two strings, returning true if they are equal modulo whitespace
+/// at the start of each line.
+fn matches_ignoring_indentation(val1: &str, val2: &str) -> bool {
+    val1.lines()
+        .zip_longest(val2.lines())
+        .all(|pair| match pair {
+            EitherOrBoth::Both(line1, line2) => line1.trim_start() == line2.trim_start(),
+            _ => false,
+        })
+}
+
 /// I001
 pub fn organize_imports(
     block: &Block,
@@ -112,8 +124,8 @@ pub fn organize_imports(
         Location::new(range.location.row(), 0),
         Location::new(range.end_location.row() + 1 + num_trailing_lines, 0),
     );
-    let actual = dedent(locator.slice_source_code_range(&range));
-    if actual == dedent(&expected) {
+    let actual = locator.slice_source_code_range(&range);
+    if matches_ignoring_indentation(actual, &expected) {
         None
     } else {
         let mut diagnostic = Diagnostic::new(UnsortedImports, range);
diff --git a/src/rules/isort/snapshots/ruff__rules__isort__tests__preserve_tabs_2.py.snap b/src/rules/isort/snapshots/ruff__rules__isort__tests__preserve_tabs_2.py.snap
new file mode 100644
index 0000000000..40d8799b10
--- /dev/null
+++ b/src/rules/isort/snapshots/ruff__rules__isort__tests__preserve_tabs_2.py.snap
@@ -0,0 +1,6 @@
+---
+source: src/rules/isort/mod.rs
+expression: diagnostics
+---
+[]
+