diff --git a/resources/test/fixtures/isort/skip.py b/resources/test/fixtures/isort/skip.py
index 02332bf21d..dbc836c643 100644
--- a/resources/test/fixtures/isort/skip.py
+++ b/resources/test/fixtures/isort/skip.py
@@ -1,10 +1,20 @@
-# isort: off
-import sys
-import os
-import collections
-# isort: on
+def f():
+    # isort: off
+    import sys
+    import os
+    import collections
+    # isort: on
 
-import sys
-import os  # isort: skip
-import collections
-import abc
+
+def f():
+    import sys
+    import os  # isort: skip
+    import collections
+    import abc
+
+
+def f():
+    import sys
+    import os  # isort:skip
+    import collections
+    import abc
diff --git a/src/directives.rs b/src/directives.rs
index 27c9eef777..e6fec89bd8 100644
--- a/src/directives.rs
+++ b/src/directives.rs
@@ -104,10 +104,13 @@ pub fn extract_isort_directives(lxr: &[LexResult]) -> IsortDirectives {
             continue;
         };
 
+        // `isort` allows for `# isort: skip` and `# isort: skip_file` to include or
+        // omit a space after the colon. The remaining action comments are
+        // required to include the space, and must appear on their own lines.
         let comment_text = comment_text.trim_end();
         if comment_text == "# isort: split" {
             splits.push(start.row());
-        } else if comment_text == "# isort: skip_file" {
+        } else if comment_text == "# isort: skip_file" || comment_text == "# isort:skip_file" {
             skip_file = true;
         } else if off.is_some() {
             if comment_text == "# isort: on" {
@@ -119,7 +122,7 @@ pub fn extract_isort_directives(lxr: &[LexResult]) -> IsortDirectives {
                 off = None;
             }
         } else {
-            if comment_text.contains("isort: skip") {
+            if comment_text.contains("isort: skip") || comment_text.contains("isort:skip") {
                 exclusions.insert(start.row());
             } else if comment_text == "# isort: off" {
                 off = Some(start);
diff --git a/src/isort/snapshots/ruff__isort__tests__skip.py.snap b/src/isort/snapshots/ruff__isort__tests__skip.py.snap
index 0be98db662..569e42ca09 100644
--- a/src/isort/snapshots/ruff__isort__tests__skip.py.snap
+++ b/src/isort/snapshots/ruff__isort__tests__skip.py.snap
@@ -5,35 +5,35 @@ expression: checks
 - kind:
     UnsortedImports: ~
   location:
-    row: 7
+    row: 12
     column: 0
   end_location:
-    row: 8
+    row: 14
     column: 0
   fix:
-    content: "import sys\n\n"
+    content: "    import abc\n    import collections\n"
     location:
-      row: 7
+      row: 12
       column: 0
     end_location:
-      row: 8
+      row: 14
       column: 0
   parent: ~
 - kind:
     UnsortedImports: ~
   location:
-    row: 9
+    row: 19
     column: 0
   end_location:
-    row: 11
+    row: 21
     column: 0
   fix:
-    content: "import abc\nimport collections\n"
+    content: "    import abc\n    import collections\n"
     location:
-      row: 9
+      row: 19
       column: 0
     end_location:
-      row: 11
+      row: 21
       column: 0
   parent: ~