diff --git a/crates/ty_python_semantic/resources/mdtest/narrow/complex_target.md b/crates/ty_python_semantic/resources/mdtest/narrow/complex_target.md
index b1a324e557..f97bc4b0f5 100644
--- a/crates/ty_python_semantic/resources/mdtest/narrow/complex_target.md
+++ b/crates/ty_python_semantic/resources/mdtest/narrow/complex_target.md
@@ -398,3 +398,84 @@ d = D()
 if d.c is not None and d.c[0].x[0] is not None:
     reveal_type(d.c[0].x[0])  # revealed: int
 ```
+
+## Narrowing with negative subscripts
+
+Narrowing should work with negative subscripts like `x[-1]`:
+
+```py
+def _(x: list[int | None]):
+    if x[-1] is not None:
+        reveal_type(x[-1])  # revealed: int
+
+def _(x: list[str | None]):
+    if x[-1] is None:
+        reveal_type(x[-1])  # revealed: None
+    else:
+        reveal_type(x[-1])  # revealed: str
+```
+
+Nested negative subscripts should also work:
+
+```py
+def _(x: list[list[int | None]]):
+    if x[-1][-1] is not None:
+        reveal_type(x[-1][-1])  # revealed: int
+```
+
+Mixed positive and negative subscripts:
+
+```py
+def _(x: list[list[int | None]]):
+    if x[0][-1] is not None:
+        reveal_type(x[0][-1])  # revealed: int
+
+    if x[-1][0] is not None:
+        reveal_type(x[-1][0])  # revealed: int
+```
+
+Attribute access combined with negative subscripts:
+
+```py
+class Container:
+    items: list[int | None]
+
+def _(c: Container):
+    if c.items[-1] is not None:
+        reveal_type(c.items[-1])  # revealed: int
+```
+
+Multiple conditions in an `and` chain:
+
+```py
+def _(x: list[int | None]):
+    # Narrowing should persist through `and` chains
+    if x[-1] is not None and x[-1] > 0:
+        reveal_type(x[-1])  # revealed: int
+```
+
+Negative indices with tuples:
+
+```py
+def _(t: tuple[int, str, None] | tuple[None, None, int]):
+    if t[-1] is not None:
+        reveal_type(t)  # revealed: tuple[None, None, int]
+    else:
+        reveal_type(t)  # revealed: tuple[int, str, None]
+
+    if t[-3] is not None:
+        reveal_type(t)  # revealed: tuple[int, str, None]
+```
+
+## Narrowing with explicit positive subscripts
+
+Narrowing should work with explicit positive subscripts like `x[+1]`:
+
+```py
+def _(x: list[int | None]):
+    if x[+0] is not None:
+        reveal_type(x[+0])  # revealed: int
+
+    if x[+1] is not None:
+        reveal_type(x[+1])  # revealed: int
+```
diff --git a/crates/ty_python_semantic/src/semantic_index/member.rs b/crates/ty_python_semantic/src/semantic_index/member.rs
index 8511c7b295..6979ac5925 100644
--- a/crates/ty_python_semantic/src/semantic_index/member.rs
+++ b/crates/ty_python_semantic/src/semantic_index/member.rs
@@ -184,6 +184,7 @@ impl MemberExpr {
                     let start_offset = path.text_len();
 
                     match &*subscript.slice {
+                        // Handle integer subscripts, like `x[0]`.
                         ast::Expr::NumberLiteral(ast::ExprNumberLiteral {
                             value: ast::Number::Int(index),
                             ..
@@ -192,6 +193,42 @@ impl MemberExpr {
                             segments
                                 .push(SegmentInfo::new(SegmentKind::IntSubscript, start_offset));
                         }
+                        // Handle negative integer subscripts, like `x[-1]`.
+                        ast::Expr::UnaryOp(ast::ExprUnaryOp {
+                            op: ast::UnaryOp::USub,
+                            operand,
+                            ..
+                        }) => match operand.as_ref() {
+                            ast::Expr::NumberLiteral(ast::ExprNumberLiteral {
+                                value: ast::Number::Int(index),
+                                ..
+                            }) => {
+                                let _ = write!(path, "-{index}");
+                                segments.push(SegmentInfo::new(
+                                    SegmentKind::IntSubscript,
+                                    start_offset,
+                                ));
+                            }
+                            _ => return None,
+                        },
+                        // Handle positive integer subscripts with explicit plus, like `x[+1]`.
+                        ast::Expr::UnaryOp(ast::ExprUnaryOp {
+                            op: ast::UnaryOp::UAdd,
+                            operand,
+                            ..
+                        }) => match operand.as_ref() {
+                            ast::Expr::NumberLiteral(ast::ExprNumberLiteral {
+                                value: ast::Number::Int(index),
+                                ..
+                            }) => {
+                                let _ = write!(path, "{index}");
+                                segments.push(SegmentInfo::new(
+                                    SegmentKind::IntSubscript,
+                                    start_offset,
+                                ));
+                            }
+                            _ => return None,
+                        },
                         ast::Expr::StringLiteral(string) => {
                             let _ = write!(path, "{}", string.value);
                             segments