diff --git a/crates/ruff_linter/resources/test/fixtures/flake8_executable/EXE003_uv_tool.py b/crates/ruff_linter/resources/test/fixtures/flake8_executable/EXE003_uv_tool.py
new file mode 100755
index 0000000000..29fb1f5013
--- /dev/null
+++ b/crates/ruff_linter/resources/test/fixtures/flake8_executable/EXE003_uv_tool.py
@@ -0,0 +1,2 @@
+#!/usr/bin/env -S uv tool run ruff check --isolated --select EXE003
+print("hello world")
diff --git a/crates/ruff_linter/resources/test/fixtures/flake8_executable/EXE003_uvx.py b/crates/ruff_linter/resources/test/fixtures/flake8_executable/EXE003_uvx.py
new file mode 100755
index 0000000000..2dbc2147bf
--- /dev/null
+++ b/crates/ruff_linter/resources/test/fixtures/flake8_executable/EXE003_uvx.py
@@ -0,0 +1,2 @@
+#!/usr/bin/env -S uvx ruff check --isolated --select EXE003
+print("hello world")
diff --git a/crates/ruff_linter/src/rules/flake8_executable/mod.rs b/crates/ruff_linter/src/rules/flake8_executable/mod.rs
index b54975e538..0e5c2d07af 100644
--- a/crates/ruff_linter/src/rules/flake8_executable/mod.rs
+++ b/crates/ruff_linter/src/rules/flake8_executable/mod.rs
@@ -22,6 +22,8 @@ mod tests {
     #[test_case(Path::new("EXE002_3.py"))]
     #[test_case(Path::new("EXE003.py"))]
     #[test_case(Path::new("EXE003_uv.py"))]
+    #[test_case(Path::new("EXE003_uv_tool.py"))]
+    #[test_case(Path::new("EXE003_uvx.py"))]
     #[test_case(Path::new("EXE004_1.py"))]
     #[test_case(Path::new("EXE004_2.py"))]
     #[test_case(Path::new("EXE004_3.py"))]
diff --git a/crates/ruff_linter/src/rules/flake8_executable/rules/shebang_missing_python.rs b/crates/ruff_linter/src/rules/flake8_executable/rules/shebang_missing_python.rs
index 3c8fecbd7a..13d6b13158 100644
--- a/crates/ruff_linter/src/rules/flake8_executable/rules/shebang_missing_python.rs
+++ b/crates/ruff_linter/src/rules/flake8_executable/rules/shebang_missing_python.rs
@@ -47,7 +47,12 @@ pub(crate) fn shebang_missing_python(
     shebang: &ShebangDirective,
     context: &LintContext,
 ) {
-    if shebang.contains("python") || shebang.contains("pytest") || shebang.contains("uv run") {
+    if shebang.contains("python")
+        || shebang.contains("pytest")
+        || shebang.contains("uv run")
+        || shebang.contains("uvx")
+        || shebang.contains("uv tool run")
+    {
         return;
     }
 
diff --git a/crates/ruff_linter/src/rules/flake8_executable/snapshots/ruff_linter__rules__flake8_executable__tests__EXE003_uv_tool.py.snap b/crates/ruff_linter/src/rules/flake8_executable/snapshots/ruff_linter__rules__flake8_executable__tests__EXE003_uv_tool.py.snap
new file mode 100644
index 0000000000..26e075ae3e
--- /dev/null
+++ b/crates/ruff_linter/src/rules/flake8_executable/snapshots/ruff_linter__rules__flake8_executable__tests__EXE003_uv_tool.py.snap
@@ -0,0 +1,4 @@
+---
+source: crates/ruff_linter/src/rules/flake8_executable/mod.rs
+---
+
diff --git a/crates/ruff_linter/src/rules/flake8_executable/snapshots/ruff_linter__rules__flake8_executable__tests__EXE003_uvx.py.snap b/crates/ruff_linter/src/rules/flake8_executable/snapshots/ruff_linter__rules__flake8_executable__tests__EXE003_uvx.py.snap
new file mode 100644
index 0000000000..26e075ae3e
--- /dev/null
+++ b/crates/ruff_linter/src/rules/flake8_executable/snapshots/ruff_linter__rules__flake8_executable__tests__EXE003_uvx.py.snap
@@ -0,0 +1,4 @@
+---
+source: crates/ruff_linter/src/rules/flake8_executable/mod.rs
+---
+