diff --git a/crates/uv-cli/src/lib.rs b/crates/uv-cli/src/lib.rs
index d9e08a813..530873112 100644
--- a/crates/uv-cli/src/lib.rs
+++ b/crates/uv-cli/src/lib.rs
@@ -2784,7 +2784,7 @@ pub struct RunArgs {
     ///
     /// Can be provided multiple times, with subsequent files overriding values defined in previous
     /// files.
-    #[arg(long, env = EnvVars::UV_ENV_FILE)]
+    #[arg(long, value_delimiter = ' ', env = EnvVars::UV_ENV_FILE)]
     pub env_file: Vec<PathBuf>,
 
     /// Avoid reading environment variables from a `.env` file.
diff --git a/crates/uv/tests/it/run.rs b/crates/uv/tests/it/run.rs
index 1ae18d843..4c36be01e 100644
--- a/crates/uv/tests/it/run.rs
+++ b/crates/uv/tests/it/run.rs
@@ -3974,12 +3974,14 @@ fn run_with_multiple_env_files() -> Result<()> {
     "###);
 
     uv_snapshot!(context.filters(), context.run().arg("test.py").env(EnvVars::UV_ENV_FILE, ".env1 .env2"), @r###"
-    success: false
-    exit_code: 2
+    success: true
+    exit_code: 0
     ----- stdout -----
+    palpatine
+    obi_wan_kenobi
+    C3PO
 
     ----- stderr -----
-    error: No environment file found at: `.env1 .env2`
     "###);
 
     Ok(())